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Hindi Journal of Regenerative MedicineISSN: 2325-9620
Goabaone Gaobotse Author
Subjects of specialization
Affiliation
Stem Cell Research, Medicinal Plants Research
Department of Biological Sciences and Biotechnology, Faculty of Science, Botswana International University of Science and Technology, P/Bag 16, Palapye, Botswana
Biography
Goabaone Gaobotse is from Department of Biological Sciences and Biotechnology and Faculty of Science in the Botswana International University of Science and Technology, P/Bag 16, Palapye, Botswana. Goabaone Gaobotse research interest are Stem Cell Research, Medicinal Plants Research.
Publications
Short Communication Open Access
Comparison of Vitronectin and Fibronectin as Substrates during the Culture of Human Embryonic Stem Cells
Author(s): Goabaone Gaobotse
The feeder free culturing of pluripotent stem cells aims to eliminate the use of mouse embryonic fibroblasts (MEFs) as substrate during in vitro culturing. Here, the potential of fibronectin and vitronectin to keep stem cells undifferentiated was compared using two different stem cell lines (HUES1 and HUES7). Cells were cultured on vitronectin and fibronectin for 3 weeks. Results showed that cells cultured in fibronectin appear to adhere slightly well initially while those in vitronectin detached and bulked up in culture. However, cells cultured on either substrate were well attached and expressed Oct4 and Nanog at the end of the 3-week protocols. This signals the capacity of both vitronectin and fibronectin to keep stem cells undifferentiated after a few passages. Such comparisons of s... view more»
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