Guido GG Author
Subjects of specialization
Affiliation
Animal Genetics and Breeding
Zoonosis Institute Dr. Luis Pasteur, Av. Diaz Velez 4821 (1405) Buenos Aires, Argentina
Mr. Guido GG, belongs to the department of Agricultural Sciences, interest in the field of Brucella canis; Urine PCR; Brucella spp urine detection; Male canine urine; Brucella canis epidemiology; Serology iELISA, from National University of Lomas Zamora, Ruta 4 Km 2,5, Pcia Buenos Aires, Argentina
Research Article Subscription
Author(s): Boeri EJ, Lopez G, Guido GG, Ciocca M, Elena SC and Wanke MM
Usefulness of Urine as a Sample for Detection of Brucella Spp in Male Canines
Urine was used as a sample and Sensitivity (S), Specificity (Sp) and the positive Likelihood Ratio (LR+) of molecular and serological methods, combined with epidemiology and the clinical symptoms for detection of Brucella spp., were compared in blood and urine samples from 241 male canines. The rapid slide agglutination test together with 2- mercaptoethanol (2-ME RSAT) were used as a screening test, followed by confirmation using an indirect immunoenzymatic assay (iELISA) and bacteriological culture. Results were as follows: Test a) PCR (Polymerase Chain Reaction) of blood compared to blood culture: S 80%, S... view moreĀ»