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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 5 Issue: 4

Usefulness of Urine as a Sample for Detection of Brucella Spp in Male Canines

Boeri EJ1*, Lopez G2, Guido GG1, Ciocca M1, Elena SC3 and Wanke MM4
1Zoonosis Institute Dr. Luis Pasteur, Av. Diaz Velez 4821 (1405) Buenos Aires, Argentina
2Faculty of Agricultural Sciences, National University of Lomas Zamora, Ruta 4 Km 2,5, Pcia Buenos Aires, Argentina
3National Health Service and Food Quality (SENASA-DILAB) OIE/ Brucellosis Reference Laboratory Talcahuano 1660 (1640), Martínez, Buenos Aires, Argentina
4Faculty of Veterinary Science, Theriogenology Area, Chorroarín 280 (1427) Buenos Aires, Argentina
Corresponding author : Eduardo J Boeri
Zoonosis Institute Dr. Luis Pasteur. Av Diaz Velez 4821 Ciudad Autónoma de Buenos Aires República, Argentina
Tel: +54 11 4958-9941
E-mail: [email protected]
Received: November 07, 2015 Accepted: May 31, 2016 Published: June 13, 2016
Citation: Boeria EJ, Lopezb G, Guidoa GG, Cioccaa M, Elena SC, et al. (2016) Usefulness of Urine as a Sample for Detection of Brucella Spp in Male Canines. J Vet Sci Med Diagn 5:4. doi:10.4172/2325-9590.1000204

Abstract

Usefulness of Urine as a Sample for Detection of Brucella Spp in Male Canines

Urine was used as a sample and Sensitivity (S), Specificity (Sp) and the positive Likelihood Ratio (LR+) of molecular and serological methods, combined with epidemiology and the clinical symptoms for detection of Brucella spp., were compared in blood and urine samples from 241 male canines. The rapid slide agglutination test together with 2- mercaptoethanol (2-ME RSAT) were used as a screening test, followed by confirmation using an indirect immunoenzymatic assay (iELISA) and bacteriological culture. Results were as follows: Test a) PCR (Polymerase Chain Reaction) of blood compared to blood culture: S 80%, Sp 92%, LR+ 10.32% (CI 5.27-19.20) test b) iELISA compared to blood culture: S 100%, Sp 94%, LR+: 16.57%) (CI 9.97-27.53), test c) PCR of urine compared to urine culture: S 100%, Sp 93% (CI 8.36-21.56), LR+: 13.64 test d) iELISA compared to urine culture: S 100%, Sp 93%, RV+: 14.5 (CI 9.03-23.26). We conclude that molecular and serological tests in conjunction with epidemiology are both useful for diagnosis and that both blood and urine samples should be assayed together.

Keywords: Brucella canis; Urine PCR; Brucella spp urine detection; Male canine urine; Brucella canis epidemiology; Serology iELISA

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