Haithem AM. Farghali4 Author

Short Communication Open Access

Free Mesenchymal Stem Cell-Associated Exosomes Induce Better Neuroregeneration than Mesenchymal Stem Cells and Neural Differentiated Mesenchymal Stem Cells in Canine Model of Spinal Cord Injury

Author(s):

Hala Gabr1 , Wael A. Elkheir2 , Amal E. Fares3*, Haithem AM. Farghali4 , Bassem E. Mahmoud5 , Mostafa A. Madbouly5 , Nehal Gamal6 , Ali M. Hamaad6 , Abo A. Elkheir6 , Rokia M. Hassan3

Aim: The aim of this study is to compare spinal cord regeneration following mesenchymal stem cell injection, neural-differentiated mesenchymal stem cells injection with that following cell free exosome injection.

 

Method: 20 dogs were randomly divided into Sham group (dorsal laminectomy only) and experimental group which were subjected to a clipping contusion of the spinal cord. One week after SCI, GFP labeled BMSCs, NSCs and MSCs-Exo were transplanted intrathecally to investigate the safety and efficacy of each one in the therapy of SCI. The effects of the transplanted cells in dogs with SCI were determined using functional neurological scoring, histopathological and immunohistochemical methods.

Results: Our data demonstrate different therapeutic app... view more»

Abstract PDF