James E. Anaissie1 Author

Research Article Open Access

Adipose Derived Stem Cell Engraftment Improves Erectile Function in a Rat Model of Cavernosal Nerve Injury

Author(s):

James E. Anaissie1 , Amit G. Reddy1 , Bryant Song2 , Zahra Brabadi3 , Nora M. Haney4, Fatemeh Daneshimehr3 , Travis Chen2 , Kevin Swan2 , Kenneth J. DeLay5 , Laith Alzweri5 , Philip Kadowitz2 , Bysani Chandrasekar6,7 , Eckhard U. Alt3 , Wayne J.G. Hellstrom4 and Reza Izadpanah3

Adipose-derived-stem-cells (ADSC) have shown promise in treating
erectile dysfunction (ED). Here we investigated the effect of ADSC
engraftment in restoring erectile function (EF) following nerve injury
during radical prostatectomy. Sprague-Dawley rats (4 groups; n=8/
group) underwent: 1) laparotomy (Lap) and immediate closure
(Sham); 2) Lap with bilateral cavernosal nerve injury (BCNI) (Crush);
3) Lap with BCNI and intracavernosal injection (ICI) of GFP+-ADSC
at surgery (INJ-1); and 4) Lap with BCNI and ICI of GFP+-ADSC
twice (at surgery and after three weeks) (INJ-2). Six weeks postBCNI, EF was measured via intracorporal pressure (ICP) response
following cavernosal nerve stimulation at 2.5V, 5V, and 7.5V. Penile
and... view more»

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