Jed Cappellazzi Author

Research Article Open Access

Microbiome Diversity of Endophytic Fungi across Latitudinal Gradients in West Coast Douglas-Fir (Pseudotsuga menziesii) Foliage

Author(s):

Hazel Daniels, Jed Cappellazzi and Jim Kiser

Research on forest microbiota lags far behind that of agricultural crops. Understanding of host-pathogen complications is exemplified in defoliation of Douglas-fir dominated forests. Although linked to Phaeocryptopus gaeumannii, etiology of the disease contrasts dramatically with previously known effects. Climate variables and seed source have been suggested as causal, however the number of potential endophytes makes the segregation of a single pathogen for needle cast problematic. A randomized block design was used to assess endophyte populations between seed sources along a latitudinal gradient among a reciprocal provenance study of Douglasfir. Needles were surface sterilized and underwent plate culturing. Isolated fungal colonies were identified through S... view more»

DOI: 10.4172/2327-4417.1000203

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