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Hindi Cell Biology: Research & TherapyISSN: 2324-9293
Kunal S Gupte Author
Subjects of specialization
Affiliation
Cellular Immunology,Metabolomics,Cell Tissue Studies,treatment of diseases,Medicine
Department of Cell therapy and Regenerative Medicine, Gujarat, India
Biography
Department of Cell therapy and Regenerative Medicine, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases &Research Centre (IKDRC)- Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS)
Publications
Research Article Subscription
Comparison of Alpha Modified Eagle’s Minimum Essential Medium with Dulbecco’s Modified Eagle’s Medium For In-Vitro Generation of Human Adipose Tissue Derived Mesenchymal Stem Cells.
Author(s): Chetan N Patel, Aruna V Vanikar*, Kunal S Gupte, Jignesh V Patel and Lovelesh A Nigam
Background: Alpha modified Eagle’s minimum essential medium (α-MEM) and Dulbecco’s modified Eagle’s medium (DMEM) are the basic media for cell culture. α-MEM has minimum glucose and amino acid concentration than DMEM. We report a study comparing the individual effect of these two media used for generation of adipose tissue derived mesenchymal stem cells (ADMSC), in terms of quantity, viability and immunophenotyping. Material and Methods: Ten grams adipose tissue resected from anterior abdominal wall of 5 willing healthy donors was divided equally into two 75 cm2 tissue culture flasks with 40 ml culture medium, one containing α-MEM and the other containing DMEM. Adipose tissue was minced into tiny pieces, incubated in collagenase-I at ... view more»
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