Lipi Poojara Author

Research Article Open Access

Development and validation of EST-SSR markers in Gymnema sylvestre R.Br.

Author(s):

Kuldeepsingh A. Kalariya, Lipi Poojara, Dipal Minipara, Parmeshwar Lal Saran, Ram Prasnna Meena, Sinora Macwan, Ponnuchamy Manivel

Known for its anti-diabetic properties, the Gymnema sylvestre belonging to the family Asclepiadaceae is native to South-Indian forests. It is also found in tropical Africa and in Australia. All arial parts of this plant contain alkaloids, flavones and saponins, but the leaves are mainly used for its medicinal properties. The diversity based on morphological, anatomical and chemo-profiles, and Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers is reported however, the genetic diversity at molecular level through the most efficient, the expressed sequence tags-SSR (EST-SSR) markers is lacking in this plant. A huge transcriptome data was generated and 5276 SSRs loci were identified in this study. The frequency of SSRs in Gymnema sylvestr... view more»

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