Mandavi Kumari Author
Subjects of specialization
Affiliation
Life Sciences,Medical Sciences,Metabolic function,treatment of diseases,Medicine
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
Mandavi Kumari is currently working in School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. He has international experience on various programs, and also contributed and participated in different countries for diverse fields of study. Research interests reflect in wide range of publications in various national and international journals.
Research Article Subscription
Author(s): Mandavi Kumari and Rajiv K Saxena
Mouse lung tissue was digested with dispase and collagenase enzymes to dissociate cells and primary lung epithelial (PLE) cells were isolated by removing leukocytes, erythroid cells, endothelial cells and fibroblasts, using a magnetic-bead based negative selection process. Purified PLE cells were cultured on fibronectin coated plastic dishes. Proliferative activity in PLE cell in culture was monitored by using time lapse live imaging technique. Proliferative activity of PLE cells started after 3 days in culture and p... view moreĀ»