Nader A Ashmawy Author

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Molecular and Biological Identification of Erwinia amylovora Egyptian Isolates Compared with Other German Strains

Author(s):

Shoeib AA, Nader A Ashmawy, Saad M Hammad and Elsayed E. Hafez

Molecular and Biological Identification of Erwinia amylovora Egyptian Isolates Compared with Other German Strains

Thirty one isolates of the enterobacterium Erwinia amylovora the causal agent of the fire blight disease were collected from different locations in Egypt and Germany. The E. amylovora (Ea) isolates were identified by biochemical tests and the obtained results were confirmed by polymerase chain reaction (PCR) using 16S universal primers. The results showed that, all the examined isolates are E. amylovora with different percentage of similarity. The phylogenetic tree constructed based on the DNA nucleotide sequences of the 16S rRNA gene revealed that the Egyp... view more»

DOI: 10.4172/2329-955X.1000156

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