Narayan Rishi Author

Short Article Open Access

Combinatorial Approach to Target Human Metapneumovirus Using shRNA and Ribozyme

Author(s):

Narayan Rishi

We aimed to restrict the propagation of human metapneumovirus (hMPV) in host cells by designing a chimeric construct encoding N gene specific shRNA linked through an intracellular cleavable linker to a ribozyme targeted to the same gene. Constructs expressing shRNA or ribozymes were also designed to evaluate their individual effects. The RNA expressed from the chimeric construct had the capability to cleave the target mRNA corresponding to N gene of hMPV under in vitro condition. Introduction of chimeric construct along with the N gene encoding DNA into A549 cells led to approximately 70% downregulation in the level of target RNA. When the construct expressing only shRNA was used, approximately 35% reduction in the level of target RNA was observed while the construct having onl... view more»

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