Nguyen TN Author

Research Article Open Access

Validation of Small Plasma Volume to Monitor HIV-1 Viral Load by Reverse Transcriptase Enzyme Activity Assay

Author(s):

Tran CT, Nguyen TN, Khuc TRH, Nguyen TKC and M Larsson

Background

Reverse transcriptase enzyme activity (RT) assay is recommended to monitor viral load (VL) for HIV-infected patients in resource-limited settings by WHO, which has many advantages such as low-cost, less technical expertise requirement, no contamination issues and excellent-concordance with the gold standard nucleic acid amplification test (NAAT). Monitoring VL in pediatric patients is still a big challenge due the large plasma volume requirement of the test. Our study aims to validate whether small plasma volume is feasible to monitor HIV-1 VL in pediatric patients by RT assay.

Materials and Methods

view more»

DOI: 10.4172/2324-8955.1000156

Abstract HTML PDF