Paul N.Fiedlerand Sandra Lobo Author

Research Article Open Access

In-house Modification and Improvement of the CDC Realtime PCR Diagnostic Assay for SARS-CoV-2 Detection

Author(s):

Srirupa Das, Candice Dowell-Martino, Lisa Arrigo, Paul N.Fiedler and Sandra Lobo

Objective: The world is currently facing an unprecedented pandemic caused by the novel coronavirus SARS-CoV-2 (COVID-19) which was first reported in late 2019 by China to the World Health Organization (WHO). The containment strategy for COVID-19, which has non-specific flu-like symptoms and where upwards of 80% of the affected has either mild or no symptoms, is critically centered upon diagnostic testing, tracking and isolation. Thus, the development of specific and sensitive diagnostic tests for COVID-19 is key towards the first successful step of disease management.
Methods: Public health organizations like the WHO and the US-based Centers for Disease Control and Prevention (CDC) have developed real-time PCR (RT-PCR) based diagnostic
tests to aid in t... view more»

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