Raffaele Caruso Author

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Electrochemical Biosensor for Interleukin-10 Detection in Real Human Plasma Patients: Heart Failure Biomedical Application

Author(s):

Abdoullatif Baraket, Michael Lee, Nadia Zine, Raffaele Caruso, Maria Giovanna Trivella and Abdelhamid Errachid

In the present study, we have analyzed the real human plasma effect for the detection of the cytokine biomarker; interleukin-10 (IL- 10) using an electrochemical impedance spectroscopy (EIS) based biosensor. IL-10 is one of the many antigens that are secreted in acute stages of inflammation after left ventricle assisted device (LVAD) implantation for patients suffering from heart failure (HF). For this interest, a biosensor was developed in order to increase the sensitivity of detection in complex medium and in the presence= of other interferences: interleukin-6 (IL-6) and interleukin-1 (IL-1) that are also secreted after LVAD implantation. The anti-human IL-10 monoclonal antibodies (anti-IL-10 mAb’s) were immobilized onto gold microelectrodes through functionalization with selfas... view more»

DOI: 10.4172/2324-8602.1000294

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