Riddhi Vyas Author

Short Communication Open Access

Incorporation of Vitreoscilla hemoglobin gene mitigates biofilm formation in Bacillus Subtilis DK1042

Author(s):

Riddhi Vyas
 

Biofilm formation is often considered as a stress combating strategy adopted by bacteria in response to a variety of cellular and environmental signals. Impaired respiration triggers biofilm formation in B. subtilis. Vitreoscilla hemoglobin (VHb) is known to supply oxygen to respiratory chain and hence improves aerobic growth and bioproduct synthesis of a variety of bacteria including Bacillus spp. Although VHb improves respiration, very little efforts have been made in elucidating its effect on biofilm formation. B. subtilis DK1042 was genetically modified to develop two integrants NRM1113 and NRM1114 containing vgb-gfp operon under 2 and 5 copies of P43 promoters, respectively, at an amyE locus by double-crossover events. Effect of VHb on biofilm formation by integrants and wild-type ... view more»

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