Sean Full Author

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Stem Cell Extracellular Matrix Interactions in Three- Dimensional System via Integrins

Author(s): Jessica M Gluck, Connor Delman, Sean Full, Richard J Shemin and Sepideh Heydarkhan-Hagvall

Stem Cell Extracellular Matrix Interactions in Three- Dimensional System via Integrins

Cells of all types interact with the extracellular matrix (ECM) through integrin-mediated interactions. Adhesion of embryonic stem (ES) cells is necessary for differentiation. We have characterized integrin expression of undifferentiated mES cells and established a system of evaluating the ECM-integrin interactions in three-dimension (3D) using electrospun scaffolds. We observed a higher proliferation rate at early time points in conventional 2D culture with various ECM proteins (collagen IV, laminin, fibronectin, vitronectin) as compared to 3D conditions. A... view more»

DOI: 10.4172/2325-9620.1000107

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