Journal of Regenerative Medicine ISSN: 2325-9620

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Research Article, J Regen Med Vol: 2 Issue: 1

Stem Cell Extracellular Matrix Interactions in Three- Dimensional System via Integrins

Jessica M Gluck1,2, Connor Delman1, Sean Full1, Richard J Shemin1, Sepideh Heydarkhan-Hagvall1*
1Cardiovascular Tissue Engineering Laboratory, Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, USA
2Department of Medicine/Cardiology, David Geffen School of Medicine, University of California, Los Angeles, USA
Corresponding author : Sepideh Heydarkhan-Hagvall, PhD
Cardiothoracic Surgery David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, 62-151 CHS, Los Angeles, CA 90095-1741, USA
Tel: 310-267-1885; Fax: 310-825-7473
E-mail: [email protected]
Received: January 16, 2013 Accepted: May 22, 2013 Published: May 27, 2013
Citation: Gluck JM, Delman C, Full S, Shemin RJ, Heydarkhan-Hagvall S (2013) Stem Cell Extracellular Matrix Interactions in Three-Dimensional System via Integrins. J Regen Med 2:1. doi:10.4172/2325-9620.1000107

Abstract

Stem Cell Extracellular Matrix Interactions in Three- Dimensional System via Integrins

Cells of all types interact with the extracellular matrix (ECM) through integrin-mediated interactions. Adhesion of embryonic stem (ES) cells is necessary for differentiation. We have characterized integrin expression of undifferentiated mES cells and established a system of evaluating the ECM-integrin interactions in three-dimension (3D) using electrospun scaffolds. We observed a higher proliferation rate at early time points in conventional 2D culture with various ECM proteins (collagen IV, laminin, fibronectin, vitronectin) as compared to 3D conditions. At later time points, we observed higher proliferations in 3D culture conditions compared to 2D culture conditions. We also detected the importance of α5, αV, β1, β5 and αVβ5 integrin subunits for cellular adhesion in 3D conditions using a cellular adhesion assay.

Keywords: Stem cells; Integrins; Extracellular matrix; Cardiovascular tissue engineering; Stem cell; scaffold; Electrospun scaffold; 3D cell culture

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