Udayasuriyan R Author

Research Article Open Access

DNA Barcoding of Freshwater Prawn Species of Two Genera Macrobrachium and Caridina Using mt-COI Gene

Author(s):

Udayasuriyan R, Saravana Bhavan P and Kalpana R

Objective: The aim was to barcoding of Macrobrachium and Caridina species through mt-COI gene, and phylogenetic reconstruction based on the degree of genetic variability between species of these two genera.

Methods: Freshwater prawns were collected from few locations in the Cauvery River, Tamil Nadu, India. They were morphologically identified as Macrobrachium lamarrei, Macrobrachium lamarrei lamarroids, Macrobrachium malcolmsonii and Caridina gracilipes. Genomic DNA was isolated and amplification of mt-COI gene was done. The nucleotide divergence and some phylogenetic information were calculated by using MEGA v.6.01... view more»

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