Journal of Immunological Techniques & Infectious Diseases ISSN: 2329-9541

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Wei Shui Author

Subjects of specialization
Aspergillus fumigatus; Headspace solid phase microextraction; Gas chromatography-mass spectrometry; Volatile organic compounds (VOCs); Breath test

Affiliation
Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China

Biography

Dr. Wei Shui was worked in the Sichuan University West China Hospital and he started his career in Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China 


Publications

Research Article Open Access

The Establishment of Realtime Fluorescent Quantitative Polymerase chain reaction (PCR) for Detection of Highly Pathogenic Avian Influenza Virus Subtype H5N1

Author(s):

Miao-lian Tan, Yuan-yuan Niu, Wei Shui, Jiancong Lin, Ming Li and Changran Zhang

Highly pathogenic strains of avian influenza virus (AIV), which are influenza A viruses, cause severe disease in domestic poultry and humans. The objective of this study was to establish a fluorescent quantitative RT-PCR assay for detection of highly pathogenic avian influenza virus (AIV) subtype H5N1. The H5 and N1 subtypespecific probe sets were developed based on avian influenza virus sequences detected in China. Two pairs of primers and two fluorescent probes were strictly designed and optimized in a reaction system. According to the amount of plasmid RNA extracted from H5N1 strains, the standard curve DWQBGWDWQBGW of fluorescent quantitative PCR was drawn and all of the specimens were then tested by means of Real-time PCR. The test of highly pathogenic ... view moreĀ»

DOI: 10.4172/2329-9541.1000154

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