Research Article, J Regen Med Vol: 4 Issue: 1
Characteristics of Human Amniotic Epithelial Cells and Bone Marrow Mesenchymal Stem Cells in a Parallel Study: Hanging Drop Colony Formation and Doubling Time
| Batool Motamedi1,2,8, Tengku Azmi Tengku Ibrahim1,3, Ahmad Bustamam Abdul4, Zeenathul Nazariah Allaudin3, Mojgan Moshrefi5, Masoomeh Hajghani6 and Seyed Noureddin Nematollahi-Mahani5,7,8* | |
| 1Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia | |
| 2Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Iran | |
| 3Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia | |
| 4Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia | |
| 5Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran | |
| 6Bahonar Hospital, Kerman University of Medical Sciences, Kerman, Iran | |
| 7Afzal Research Institute (NGO), Kerman, Iran | |
| 8Kerman Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran | |
| Corresponding author : Seyed Noureddin Nematollahi-Mahani Kerman Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Avecina St., Tahmasbabad Cross, Kerman, Iran Tel: +98-341-3221666 E-mail: nnematollahi@kmu.ac.ir; nematollahimahani@yahoo.com |
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| Received: February 13, 2015 Accepted: May 29, 2015 Published: June 06, 2015 | |
| Citation: Motamedi B, Ibrahim TAT, Abdul AB, Allaudin ZN, Moshrefi M, et al. (2015) Characteristics of Human Amniotic Epithelial Cells and Bone Marrow Mesenchymal Stem Cells in a Parallel Study: Hanging Drop Colony Formation and Doubling Time. J Regen Med 4:1. doi:10.4172/2325-9620.1000120 |
Abstract
Characteristics of Human Amniotic Epithelial Cells and Bone Marrow Mesenchymal Stem Cells in a Parallel Study: Hanging Drop Colony Formation and Doubling Time
Amniotic epithelial cells exhibit pluripotent capacity with some unique properties. They are considered as a good candidate in regenerative medicine. Human bone marrow mesenchymal stromal cells (hBM-MSC) are the gold standard in cell therapy procedures, but whether these cells could be replaced with hAEC remains a debate? We compared the stem cell characteristics of hAEC with hBM-MSC considering isolation, morphology, population doubling time, colony formation on substratum and in suspension, alkaline phosphatase activity and expression of some CD Markers. hAEC were round and cubical but hBM-MSC appeared spindle-shaped in culture. Both groups of cells expressed mesenchymal stem cells markers (CD44, CD90) and they did not express CD34. OCT4 was highly expressed in hAEC but not in hBM-MSC. Their proliferation rate was slower than hBM-MSC. Either of the cell groups could form colonies on substratum as well as suspension with a positive alkaline phosphatase activity. Our results showed that biological properties of human amniotic epithelial cells are to some extent similar to human bone marrow mesenchymal cells.
