Journal of Regenerative Medicine ISSN: 2325-9620

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Research Article, J Regen Med Vol: 4 Issue: 1

Characteristics of Human Amniotic Epithelial Cells and Bone Marrow Mesenchymal Stem Cells in a Parallel Study: Hanging Drop Colony Formation and Doubling Time

Batool Motamedi1,2,8, Tengku Azmi Tengku Ibrahim1,3, Ahmad Bustamam Abdul4, Zeenathul Nazariah Allaudin3, Mojgan Moshrefi5, Masoomeh Hajghani6 and Seyed Noureddin Nematollahi-Mahani5,7,8*
1Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
2Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Iran
3Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
4Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
5Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
6Bahonar Hospital, Kerman University of Medical Sciences, Kerman, Iran
7Afzal Research Institute (NGO), Kerman, Iran
8Kerman Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Corresponding author : Seyed Noureddin Nematollahi-Mahani
Kerman Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Avecina St., Tahmasbabad Cross, Kerman, Iran
Tel: +98-341-3221666
E-mail: [email protected]; [email protected]
Received: February 13, 2015 Accepted: May 29, 2015 Published: June 06, 2015
Citation: Motamedi B, Ibrahim TAT, Abdul AB, Allaudin ZN, Moshrefi M, et al. (2015) Characteristics of Human Amniotic Epithelial Cells and Bone Marrow Mesenchymal Stem Cells in a Parallel Study: Hanging Drop Colony Formation and Doubling Time. J Regen Med 4:1. doi:10.4172/2325-9620.1000120

Abstract

Characteristics of Human Amniotic Epithelial Cells and Bone Marrow Mesenchymal Stem Cells in a Parallel Study: Hanging Drop Colony Formation and Doubling Time

Amniotic epithelial cells exhibit pluripotent capacity with some unique properties. They are considered as a good candidate in regenerative medicine. Human bone marrow mesenchymal stromal cells (hBM-MSC) are the gold standard in cell therapy procedures, but whether these cells could be replaced with hAEC remains a debate? We compared the stem cell characteristics of hAEC with hBM-MSC considering isolation, morphology, population doubling time, colony formation on substratum and in suspension, alkaline phosphatase activity and expression of some CD Markers. hAEC were round and cubical but hBM-MSC appeared spindle-shaped in culture. Both groups of cells expressed mesenchymal stem cells markers (CD44, CD90) and they did not express CD34. OCT4 was highly expressed in hAEC but not in hBM-MSC. Their proliferation rate was slower than hBM-MSC. Either of the cell groups could form colonies on substratum as well as suspension with a positive alkaline phosphatase activity. Our results showed that biological properties of human amniotic epithelial cells are to some extent similar to human bone marrow mesenchymal cells.

Keywords: Human amniotic epithelial cells; Human bone marrow mesenchymal stem cells; Population doubling time; Colony formation assay; Alkaline phosphatase

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