Journal of Virology & Antiviral ResearchISSN: 2324-8955

Research Article, J Virol Antivir Res Vol: 3 Issue: 1

Comparison of Real-Time RTPCR Assay with Direct Sequencing for Detection of Sensitivity or Resistant to Oseltamivir in Influenza A/H3N2 Viruses

Mohammad Hadi Karbalaie Niya, Jila Yavarian, Nazanin Zahra Shafiei Jandaghi, Shohreh Shahmahmoodi and Talat Mokhtari Azad*
School of Public Health, Tehran University of Medical Sciences
Corresponding author : Dr. Talat Mokhtari Azad
Virology department, School of Public Health, Tehran University of Medical Sciences, Iran
Tel: +98(21)88962343
E-mail: [email protected]
Received: March 18, 2014 Accepted: May 21, 2014 Published: May 23, 2014
Citation: KarbalaieNiya MH, Yavarian J, ShafieiJandaghi NZ, Shahmahmoodi S, MokhtariAzad T, et al. (2014) Comparison of Real-Time RT-PCR Assay with Direct Sequencing for Detection of Sensitivity or Resistant to Oseltamivir in Influenza A/H3N2 Viruses. J Virol Antivir Res 3:1. doi:10.4172/2324-8955.1000120

Abstract

Comparison of Real-Time RTPCR Assay with Direct Sequencing for Detection of Sensitivity or Resistant to Oseltamivir in Influenza A/H3N2 Viruses

Background: Currently, there is an increasing risk of a worldwide influenza A virus epidemics not only influencing livestock but also humans, then research about the prophylaxis and treatment of these viruses and detection of drug resistant mutants is a must. Nowadays Oseltamivir “the neuraminidase (NA) inhibitor” is the suitable drug for preventing and treatment of these virus infections. Drug resistant viruses has already detected by mutations of the enzyme's active site in the neuraminidase gene that cause amino acid substitutions predominantly at positions like 119, 274, 292. The objective of this research was the comparison of the Real-time RT-PCR assay with direct sequencing for detection of sensitivity or resistant to Oseltamivir in Influenza A/H3N2 viruses.

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