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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 3 Issue: 4

Development of VP6 Gene Specific Reverse Transcription(RT)-PCR Assay for Detectionof Avian Group D Rotavirus in Diarrheic Chickens

Jobin Jose Kattoor1, Yashpal Singh Malik1*, Naveen Kumar1, Kuldeep Sharma1, Shubhankar Sircar1, Munish Batra2, Kuldeep Dhama3 and Raj Kumar Singh4
1Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
2Department of Veterinary Pathology, GB Pant University of Agriculture and Technology, Udham Singh Nagar, Uttarakhand, India
3Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
4Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
Corresponding author : Yashpal Singh Malik
Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
Tel: +91-581-2302777; Fax: +91-581-2301757
E-mail: [email protected]
Received: February 25, 2014 Accepted: June 27, 2014 Published: July 02, 2014
Citation: Kattoor JJ, Malik YS, Kumar N, Sharma K, Sircar S, et al., (2014) Development of VP6 Gene Specific Reverse Transcription (RT)-PCR Assay for Detection of Avian Group D Rotavirus in Diarrheic Chickens. J Vet Sci Med Diagn 3:3. doi:10.4172/2325-9590.1000145

Abstract

 Development of VP6 Gene Specific Reverse Transcription(RT)-PCR Assay for Detection of Avian Group D Rotavirus in Diarrheic Chickens

The group D rotavirus (RVD) is an evolving viral cause of acute gastroenteritis in avian species world over. Despite the increase in the frequency of avian RVD infections world over, isolates from Indian poultry have yet to be characterised. Paucity of epidemiological studies with unavailability of sensitive and rapid detection methods for RVD in poultry led to design of the present study, aiming at development and validation of a RT-PCR assay, targeting the conserved group specific region of VP6 gene of RVD, for virus detection purpose.

Keywords: Avian group-D rotavirus; RT-PCR; VP6 gene; Diagnosis; India

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