Journal of Clinical & Experimental OncologyISSN: 2324-9110

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Research Article, J Clin Exp Oncol Vol: 3 Issue: 1

Evaluation of Plasma microRNA Expression Levels in Early Diagnosis of Colorectal Cancer

Serin Akbayir1*, Nil Dogruer Unal2, Senay Balcı1, Aysegul Gorur1, Hatice Yıldırım Yaroglu3, Lokman Ayaz4, Ramazan Gundogdu5, Musa Dırlık5, Mehmet Sami Serın6,7 And Lulufer Tamer1
1Department of Biochemistry, Mersin University Faculty of Medicine, Mersin 33079, Turkey
2Department of Biochemistry, Mersin University Faculty of Pharmacy, Mersin 33169, Turkey
3School of Health Sciences, Toros University, Mersin 33140, Turkey
4Department of Biochemistry, Trakya University Faculty of Pharmacy, Edirne 22030, Turkey
5Department of General Surgery, Faculty of Medicine, Mersin University, Mersin 33079, Turkey
6Department of Pharmaceutical Microbiology, Mersin University Faculty of Pharmacy, Mersin 33169, Turkey
7Technology Education, Research and Application Center, Mersin University, 33169, Mersin, Turkey
Corresponding author : Serin Akbayir
Department of Biochemistry, Faculty of Medicine, Mersin University, Mersin, 33079, Turkey
Tel: +90-324-3374300/1530; Fax: +90-324 337 43 05
E-mail: [email protected]
Received: December 14, 2013 Accepted: February 24, 2014 Published: February 28, 2014
Citation: Akbayir S, Unal ND, Balci S, Gorur A, Yaroglu HY, et al. (2014) Evaluation of Plasma microRNA Expression Levels in Early Diagnosis of Colorectal Cancer. J Clin Exp Oncol 3:1. doi:10.4172/2324-9110.1000119

Abstract

Evaluation of Plasma microRNA Expression Levels in Early Diagnosis of Colorectal Cancer

 Background: Colorectal cancer (CRC) is the third most frequently diagnosed cancer all over the world and the prognosis is associated with the stage at diagnosis. Due to insufficient sensitivity of tumor markers, as CEA, CA19.9, guaiac-based fecal occult blood and fecal immunochemical occult blood testing used in the routine, there is a need for non-invasive, new biomarkers with high sensitivity and specificity and they will be helpful in early diagnosis. microRNAs (miRNA) are small, single-stranded noncoding transcripts, and some of them play critical roles in tumorigenesis and have a potentially clinical value in diagnosis, treatment and prognosis evaluation for CRC. In this study we aimed to compare miRNA’s expression profiles between pathologically diagnosed CRC patients and healthy control groups.
Methods: Blood samples from participants were collected for test-scale analysis. Plasma samples were extracted from whole blood, then total RNA are isolated and pre-amplified as manufacturer’s protocol. Finally, real-time PCR step was performed with BioMark System by using manufacturer’s protocol.
Results: Plasma samples of 37 CRC patients and 238 healthy subjects were examined to profiling 741 miRNAs expression, using high-throughput real-time quantitative RT-PCR. Among them, 3 miRNAs (miR-1274A, miR- 875-5p, miR-34c-5p) were found significantly up-regulated while 8 miRNAs (miR-30d-5p, miR-150-5p, miR-30a-5p, miR-34a-5p, miR-223-3p, miR-576- 3p, miR-30c-5p, miR-195-5p) were found down-regulated in CRC patients compared to control group.
Conclusion: In this study it was shown that miR-150-5p, miR-30a-5p, miR- 34a-5p, and miR-195-5p can be helpful in early diagnosis of CRC similar to some other studies.

Keywords: Colorectal cancer; Early diagnosis; MicroRNAs; Real-Time PCR; Tumor marker

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