Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 3 Issue: 1

Flow-Cytometric Analysis of Testicular Germ Cell Populations in Rams (Ovis Aries)

Divya V1, Girish Kumar V1, Nandi S2*, Ramchandra SG3 and William Rasican Surin4
1Department of Veterinary Biochemistry, Veterinary College, KVAFSU, Hebbal, Bangalore, India
2National Institute of Animal Nutrition and Physiology (NIANP), Adugodi, Bangalore, India
3Central Animal Facility, Indian Institute of Science, Bangalore, India
4Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India
Corresponding author : Nandi S
National Institute of Animal Nutrition and Physiology (NIANP) Adugodi, Bangalore-560030, India
E-mail: [email protected]
Received: September 24, 2013 Accepted: December 11, 2013 Published: December 16, 2013
Citation: Divya V, Girish Kumar V, Nandi S, Ramchandra SG,Surin WR (2014) Flow-Cytometric Analysis of Testicular Germ Cell Populations in Rams (Ovis Aries). J Vet Sci Med Diagn 3:1. doi:10.4172/2325-9590.1000126

Abstract

Flow-Cytometric Analysis of Testicular Germ Cell Populations in Rams (Ovis Aries)

The present study was conducted to analyse the germ cell population in testis of rams of different age groups and in wethers by flow cytometric technique and to determine the mitotic index and dihydrotestosterone level in different age groups of rams and in wethers. The germ cells in the testis of all the rams of the different group was analyzed and quantified by flow cytometry after staining with propidium iodide.

Keywords: Flow-cytometry; Germ cell; Rams (Ovis aries)

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