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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 4 Issue: 4

Molecular Identification of Nosema ceranae and Nosema apis in Native Bulgarian Honey Bee (Apis mellifera rodopica)

Hristov PI1*, Rositsa S2, Bojko N 1 and Georgi R 1
1Department of Animal Diversity and Resources, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, “acad. G. Bonchev” str. Bl. 25, Sofia, 1113, Bulgaria
2Stockbreeding and Agricultural Experimental Station, 35 “Nevyastata” str., Smolyan, 4700, Bulgaria
Corresponding author : Peter Hristov
Department of Animal Diversity and Resources, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, “acad. G. Bonchev” str. Bl. 25, Sofia, 1113, Bulgaria
Tel: + 359 2 979 2327
E-mail: [email protected]
Received: April 27, 2015 Accepted: May 08, 2015 Published: May 13, 2015
Citation: Hristov PI, Rositsa S, Bojko N , Georgi R (2015) Molecular Identification of Nosema ceranae and Nosema apis in Native Bulgarian Honey Bee (Apis mellifera rodopica). J Vet Sci Med Diagn 4:4. doi:10.4172/2325-9590.1000165

Abstract

Molecular Identification of Nosema ceranae and Nosema apis in Native Bulgarian Honey Bee (Apis mellifera rodopica)

The aim of current study is to investigate the presence of two main microsporidian parasites N. apis and N. ceranae in Bulgarian local honey bee. The discrimination among two Nosema species was carried out on 18 individual samples. The 16S (SSU) rDNA gene region was analyzed and duplex PCR assay was performed. The results showed (1) the dominance of N. ceranae infection in Bulgaria (2) N. ceranae most likely switched its host to A. mellifera, probably within the last decade (3) N. ceranae is now a parasite on A. mellifera across worldwide. The rapid, long-distance dissemination of N. ceranae is likely due to transport of infected honey bees and/or by increased mobility of people, goods and livestock.

Keywords: Apis mellifera rodopica; Nosematosis; Duplex PCR assay

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