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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 2 Issue: 4

Optimal Methods for Blood Collection and RNA Extraction in Horses

Fahad Raza1,2, Yung Fu Chang1, Thomas J Divers3 and Hussni O Mohammed1*
1Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca NY, 14853, USA
2Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, PMAS Arid Agriculture University, Rawalpindi, Pakistan
3Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca NY, 14853, USA
Corresponding author : Hussni O Mohammed
S1-070 Shurman Hall, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
Tel: (607) 253-3566; Fax: (607) 253-3083
E-mail: [email protected]
Received: September 25, 2013 Accepted: November 04, 2013 Published: November 08, 2013
Citation: Raza F, Chang YF, Divers TJ, Mohammed HO (2013) Optimal Methods for Blood Collection and RNA Extraction in Horses. J Vet Sci Med Diagn 2:4. doi:10.4172/2325-9590.1000125

Abstract

Optimal Methods for Blood Collection and RNA Extraction in Horses

Detecting gene expression through RNA is a complex yet very effective mechanism to investigate functionality of the genome and has a widespread potential application in modern equine research. Degradation of RNA after blood collection is a major challenge and requires that sample be subjected to appropriate storage and cautious handling before and during processing. The current study was designed to optimize and evaluate a protocol for RNA extraction from horse blood and to establish the role of incubation time and temperature on RNA purity and yield.

Keywords: RNA extraction; Quality; Purity; Stabilized blood; Horse

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