Research Article, J Vet Sci Med Diagn Vol: 2 Issue: 4
Optimal Methods for Blood Collection and RNA Extraction in Horses
| Fahad Raza1,2, Yung Fu Chang1, Thomas J Divers3 and Hussni O Mohammed1* | |
| 1Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca NY, 14853, USA | |
| 2Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, PMAS Arid Agriculture University, Rawalpindi, Pakistan | |
| 3Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca NY, 14853, USA | |
| Corresponding author : Hussni O Mohammed S1-070 Shurman Hall, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA Tel: (607) 253-3566; Fax: (607) 253-3083 E-mail: hom1@cornell.edu |
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| Received: September 25, 2013 Accepted: November 04, 2013 Published: November 08, 2013 | |
| Citation: Raza F, Chang YF, Divers TJ, Mohammed HO (2013) Optimal Methods for Blood Collection and RNA Extraction in Horses. J Vet Sci Med Diagn 2:4. doi:10.4172/2325-9590.1000125 |
Abstract
Optimal Methods for Blood Collection and RNA Extraction in Horses
Detecting gene expression through RNA is a complex yet very effective mechanism to investigate functionality of the genome and has a widespread potential application in modern equine research. Degradation of RNA after blood collection is a major challenge and requires that sample be subjected to appropriate storage and cautious handling before and during processing. The current study was designed to optimize and evaluate a protocol for RNA extraction from horse blood and to establish the role of incubation time and temperature on RNA purity and yield.
