Journal of Virology & Antiviral ResearchISSN: 2324-8955

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article, J Virol Antivir Res Vol: 7 Issue: 1

Cathepsin B Plays a Key Role in Optimal Production of the Influenza A- Virus

Macon D Coleman, Soon-Duck Ha, Mansour Haeryfar, Stephen Dominic Barr and Sung Ouk Kim*

Department of Microbiology and Immunology and Center for Human Immunology, Drake Research Institute, Western University, London, Ontario, Canada

*Corresponding Author : Sung Ouk Kim
Department of Microbiology and Immunology and Center for Human Immunology, Siebens-7 Drake Research Institute, Western University, Ontario, Canada
Tel: (519)850-2961
Fax: (519)-661-2046
E-mail: [email protected]

Received: November 24, 2017 Accepted: May 04, 2018 Published: May 11, 2018

Citation: Coleman MD, Ha S, Haeryfar M, Barr SD, Kim SO (2018) Cathepsin B Plays a Key Role in Optimal Production of the Influenza A- Virus. J Virol Antivir Res 7:1. doi: 10.4172/2324-8955.1000178

Abstract

Background: Influenza A- virus (IAV) is the etiologic agent of the febrile respiratory illness, commonly referred to as ‘flu’. The lysosomal protease cathepsin B (CTSB) has shown to be involved in the lifecycle of various viruses. Here, we examined the role of CTSB in the IAV lifecycle. 

Methods: CTSB-deficient (CTSB -/-) macrophages and the human lung epithelial cell line A549 cells treated with CA-074Me were infected with the A/Puerto Rico/8/34 strain of IAV (IAV- PR8). Viral
entry and propagation were measured through quantitative realtime RT-PCR; production and localization of hemagglutinin (HA) protein in the infected host cells were analysed by Western blots, flow cytometry and confocal microscopy; production of progeny viruses were measured by a hemagglutination assay. 

Results: CTSB -/- macrophages and CA-074Me-treated A549 cells had no defects in incorporating IAV-PR8 virions and permitting viral RNA synthesis. However, these cells produced significantly lower amounts of HA protein and progeny virions than wild-type or untreated cells. 

Conclusion: These data indicate that CTSB is involved in the expression of IAV-PR8 HA protein and subsequent optimal production of IAV-PR8 progeny virions. Targeting CTSB can be a novel therapeutic strategy for treating IAV infection.

Keywords: Cathepsin B; Influenza A virus; CA-074 Me; Hemagglutinin

Track Your Manuscript

Share This Page

Media Partners