International Journal of Ophthalmic PathologyISSN: 2324-8599

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Research Article, Int J Ophthalmic Pathol Vol: 6 Issue: 1

Cytomorphology of Conjunctival Epithelium in Ocular Surface Disorders

Satyaprakash Singh, Chandraprakash Oli*, Vatasala Mishra, Deepika Singh, Jagriti Rana and Arun Singh
Department of Ophthalmology and Department of Pathology, Motilal Nehru Medical College, Regional Institute of Ophthalmology at Government MD Eye Hospital, Allahabad, Uttar Pradesh, India
Corresponding author : Dr. Chandraprakash Oli
Room no 207, New PG Boys Hostel, SRN Hospital Campus, Allahabad, Uttar Pradesh- 211001, India
Tel: +919545261984
Received: December 08, 2016 Accepted: January 07, 2017 Published: January 12, 2017
Citation: Singh S, Oli C, Mishra V, Singh D, Rana J, et al. (2017) Cytomorphology of Conjunctival Epithelium in Ocular Surface Disorders. Int J Ophthalmic Pathol 6:1. doi: 10.4172/2324-8599.1000195


Introduction: To study cytomorphological changes in various ocular surface disorders and find out the sensitivity, specificity and the diagnostic accuracy of impression cytology.
Materials and Methods: One hundred and ten patients (84 cases and 26 controls) were included in this study. Out of these 84 cases, 25 cases having vitamin A deficiency (VAD), 19 cases are having vernal keratoconjunctivitis (VKC), 30 of dry eye and 10 of chemical burn. Impression cytology was performed with each patient and the results were noted. Biopsy was done in 2 patients from each group. Patients were examined for goblet cell density in the form of clumping of goblet cells and degree of squamous metaplasia in terms of loss of cell cohesion, altered cell morphology and keratinisation.
Results: On impression cytology, all cases of vitamin A deficiency showed loss of goblet cells and 92% showed squamous metaplasia. In case of vernal conjunctivitis showed clumping (37%), loss of goblet cells (63%) and squamous metaplasia (64%). In dry eye group, severe reduction of goblet cells (73%) with moderate to severe grade of squamous metaplasia (88%) was seen. All cases of chemical burn showed marked reduction of goblet cells and squamous metaplasia. Control group, showed mild reduction in goblet cells (23%) and mild grade of squamous metaplasia (15%). Sensitivity, specificity and diagnostic accuracy of impression cytology was 89.28%, 76.92% and 86.36% respectively. Cytological findings are well correlated with histological findings.
Conclusion: Impression cytology was found to be good technique for diagnosing metaplastic changes in various ocular surface disorders.

Keywords: Impression cytology; Vitamin-A deficiency; Vernal keratoconjunctivitis; Dry eye; Chemical injury of eye

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