Journal of Clinical & Experimental OncologyISSN: 2324-9110

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Research Article, J Clin Exp Oncol Vol: 4 Issue: 4

Effect of Vaccination with Different Types and Dosages against a Very Virulent Marek’s Disease Virus Strain

Zhenhua Gong1*, Kang Zhang2*, Lei Li1, Yuan Qiu1, Jinping Li1, Guangyu Hou1, Jianmin Yu1, Jianlin Wang2, Hu Shan2 and Hongwei Wang3
1China Animal Healthy and Epidemic Center, Qingdao 266032, China
2Qingdao Agriculture University, Qingdao 266109, China
3Zhucheng Animal Husbandry and Veterinary Administration, Weifang 262200,China
Corresponding author's : Zhenhua Gong
China Animal Healthy and Epidemic Center, Qingdao 266032, China
Tel: 0086-135-0532-1832
E-mail: [email protected]
Kang Zhang
Qingdao Agriculture University, Qingdao 266109, China
Tel: 0086-532-8562-8464;
E-mail: [email protected]
Received: October 22, 2015 Accepted: November 19, 2014 Published: December 14, 2015
Citation: Gong Z, Zhang K, Li L, Qiu Y, Li J, et al. (2015) Effect of Vaccination with Different Types and Dosages against a Very Virulent Marek’s Disease Virus Strain. J Clin Exp Oncol 4:4. doi:10.4172/2324-9110.1000147

Abstract

Effect of Vaccination with Different Types and Dosages against a Very Virulent Marek’s Disease Virus Strain

Abstract

In this study, Marek’s disease(MD) vaccines including herpesvirus of turkeys (HVT) vaccine, attenuated serotype 1 Marek’s disease virus (MDV) CVI988 vaccine and nonpathogenic serotype 2 MDV (SB1 strain) culture were used individually or jointly to immunize 1-day-old specific pathogen free (SPF) chickens at different dosages, the immunized chickens were intradermally infected with 2000 plaque forming unit (PFU) of duck embryo fibroblast (DEF) passaged SD2012-1, a very virulent Marek’s disease virus. All chickens of the experiments and control chickens were observed daily throughout the entire experimental period to assess the vaccines efficacy and immunization dose against MDV SD2012- 1 Strain. CVI988, CVI988+HVT and CVI988+SB1+HVT could partially protect chickens against Marek’s disease after infection with SD2012-1. CVI988+HVT and CVI988+SB1+HVT could provide more efficient protection than CVI988 at normal and high immunization dosage. The protection was not fully satisfactory, and vaccines used at high dosage could not provide more than PI 65. Excessive dilution of these vaccines could result in obvious decrease on protection against SD2012-1. In this study, 450 feather pulp samples collected from the dead or living chickens on Day 150 were detected to be MDV positive by polymerase chain reaction (PCR), and the positive rate of MDV was 100%.

Keywords: Marek’s disease virus; Challenge; Virulence; Vaccine

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