Journal of Regenerative Medicine ISSN: 2325-9620

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Research Article, J Regen Med Vol: 8 Issue: 1

Human Bone Marrow-Derived Mesenchymal Stromal Cells Injected Intrathecally in NOD/SCID Mice Preclinical Study of Biosafety and Biodistribution

Mari Paz Quesada1*, David García-Bernal1,2, Diego Pastor3, Alicia Estirado4, Miguel Blanquer1,2, Ana M García-Hernández1, José M Moraleda1,2 and Salvador Martínez4,5,6

1Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, Biomedical Research Institute of Murcia, IMIB-Arrixaca, Campus of International Excellence “Campus Mare Nostrum” University of Murcia, Murcia, Spain

2Internal Medicine Departments, Medicine School, University of Murcia, Murcia, Spain

3Sport Research Center, University Miguel Hernández of Elche, Alicante, Spain

4Neuroscience Institute UMH-CSIC, University Miguel Hernández of Elche, Alicante, Spain

5CIBERSAM-ISCIII, Spain

6Human Anatomy Department, Medicine School, University Miguel Hernández of Elche, Alicante, Spain

*Corresponding Author : Mari Paz Quesada
Cellular Therapy and Hematopoietic Transplant Unit, Haematology Department IMIB-Arrixaca (Spain), 30120 El Palmar (Murcia), Spain
Tel: 868 885 013
E-mail: [email protected]

Received: January 28, 2019 Accepted: February 13, 2019 Published: February 20, 2019

Citation: Quesada MP, García-Bernal D, Pastor D, Estirado A, Blanquer M, et al. (2019) Human Bone Marrow-Derived Mesenchymal Stromal Cells Injected Intrathecally in NOD/SCID Mice Preclinical Study of Biosafety and Biodistribution. J Regen Med 8:1. doi: 10.4172/2325-9620.1000147

Abstract

Objective: Mesenchymal stromal cells (MSCs) have potent immunomodulatory and neuroprotective properties, and have been tested in neurodegenerative diseases resulting in meaningful clinical improvements. Regulatory guidelines specify the need to perform preclinical studies prior any clinical trial, including bio-distribution assays and tumourigenesis exclusion. We conducted a preclinical study of human bone marrow MSCs (hBM-MSCs) injected by intrathecal route in immunosuppressed NOD/SCID mice, to explore cellular bio-distribution and toxicity, as a privileged administration method for cell therapy in Friedreich´s Ataxia (FRDA). Methods: For this purpose, hBM-MSCs have been characterized according to the ISCT standards and 3 × 105 cells were injected by intrathecal route in 12 animals (experimental group) and the same volume of culture media in 6 animals (control group). Blood samples were collected at 24 hours (n=9) or 4 months (n=9) to assess toxicity, and eight organs were harvested for histology studies. Genomic DNA was isolated from all tissues, and mouse GAPDH and human β2M and β-actin genes were amplified by qPCR to analyze hBMMSCs biodistribution. Results: There were no deaths or acute or chronic toxicity. Hematology, biochemistry and body weight were in the range of normal values in all groups. At 24 hours hBM-MSCs were detected in 4/6 spinal cords and 1/6 hearts, and at 4 months in 3/6 hearts and 1/6 brains of transplanted mice. No tumours were found. Conclusion: This study demonstrated that intrathecal injection of hBM-MSCs is safe, non-toxic and do not produce tumors. These results provide further evidence that hBM-MSCs might be used in a clinical trial in patients with FRDA.

Keywords: Cell therapy; Intrathecal transplantation; Bone marrow-derived mesenchymal stromal cells; Neuroprotection; preclinical study; Friedreich┬┤s Ataxia

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