Journal of Pharmaceutics & Drug Delivery Research ISSN: 2325-9604

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Research Article, J Pharm Drug Deliv Res Vol: 7 Issue: 2

In vitro Cell Compatibility of Prepared Collagen–Glycosaminoglycans–Selenium Substituted Hydroxyapatite Nanopowders Composite Scaffolds

Sara Ibrahim Korowash1*, Anna Burdzinska2, Emilia Choinska3, Amany Abdel-Moniem Mostafa1 and Doreya Mohamed Ibrahim1

1Ceramics Department, National Research Centre, Dokki, Cairo, Egypt

2Department of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, Nowogrodzka 59, Warsaw, Poland

3Warsaw University of Technology, Warsaw, Poland

*Corresponding Author : Sara Ibrahim Korowash
Ceramics Department, National Research Centre, 12622 Dokki, Cairo, Egypt
Tel: +202 33371564
E-mail: sara_ [email protected]

Received: December 03, 2018 Accepted: December 18, 2018 Published: December 18, 2018

Citation: Korowash SI, Burdzinska A, Choinska E, Mostafa AA, Ibrahim DM (2018) In vitro Cell Compatibility of Prepared Collagen–Glycosaminoglycans–Selenium Substituted Hydroxyapatite Nanopowders Composite Scaffolds. J Pharm Drug Deliv Res 7:2. doi: 10.4172/2325-9604.1000183

Abstract

Scaffolds for hepatic tissue engineering were successfully prepared by freeze-drying technique including four types namely; collagenchondroitin sulfate (Co-CS), collagen-sodium hyalurnate (Co-SH), collagen-chondroitin sulfate-selenium substituted hydroxyapatite nanopowders (Co-CS-SeHA2) and collagen-sodium hyalurnateselenium substituted hydroxyapatite nanopowders (Co-SHSeHA2) and using gluteraldehyde as cross-linker to increase the scaffolds strength. The formed scaffolds were characterized by SEM and the results showed porous structured with desirable pore sizes ranging from 73.7 to 103 nm, for hepatocytes culture. The cell compatibility was tested using human bone marrow mesynchymal stem cells (BM-MSCs). Results of fluorescent microscopy of the scaffolds showed that the highest number of cells was on Co-CS-SeHA2 scaffold. The cells were distributed in groups which suggested they proliferated on this material. The Co-CS-SeHA2 prepared scaffold can be applied for further liver/ hepatocytes studies.

Keywords: Selenium, hydroxyapatite; Nanoparticles; Collagen; Chondroitin sulfate; Sodium hyalurnate; Glycosaminoglycans; Scaffolds; Liver; Bone marrow mesenchymal stem cells

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