Journal of Veterinary Science & Medical DiagnosisISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 5 Issue: 2

Intrauterine Infection by Mycobacterium avium subsp. paratuberculosis in buffalo (Bubalus bubalis)

Belo-Reis AS1*, Salvarani FM1, Brito MF2, Fonseca AA3, Silva NS6, Silveira JAS1, Reis JKP4, Silva JB5, Oliveira CMC1 and Barbosa JD1
1Institute of Veterinary Medicine, Federal University of Para, PA 68740-000 Castanhal, Brazil
2Department of Epidemiology and Public Health, Veterinary Institute, Rural Federal University of Rio de Janeiro (UFRRJ ) Seropedica, RJ 23890-000, Brazil
3National Agricultural Laboratory of Minas Gerais (Lanagro), Ministry of Agriculture, Livestock and Supply (MAPA), Pedro Leopoldo, MG 36000-000, Brazil
4Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais (UFMG ), Belo Horizonte, MG 30123-970, Brazil
5Imunoparasitologia Laboratory, Department of Veterinary Pathology, Faculty of Agricultural Sciences and Veterinary FCAV - UNESP, SP 14884-900, Jaboticabal, Brazil
6Federal Rural University of Amazonia, Paragominas, PA 68627-451, Brazil
Corresponding author : Alessandra S. Belo-Reis
Institute of Veterinary Medicine, Federal University of Para, PA 68740-000 Castanhal, Brazil
Tel: +55 9181469062
E-mail: alessandra_belo22@hotmail.com
Received: November 05, 2015 Accepted: February 18, 2016 Published: February 23, 2016
Citation: Belo-Reis AS, Salvarani FM, Brito MF, Fonseca AA, Silva NS, et al. (2016) Intrauterine Infection by Mycobacterium avium subsp. paratuberculosis in buffalo (Bubalus bubalis). J Vet Sci Med Diagn 5:2. doi:10.4172/2325-9590.1000189

Abstract

Intrauterine Infection by Mycobacterium avium subsp. paratuberculosis in buffalo (Bubalus bubalis)

This study reports the detection of Mycobacterium avium subsp. paratuberculosis (Map) in the uterus and fetus of a buffalo (Bubalus bubalis). Samples of the buffalo cow’s ileum, mesenteric lymph node, uterus and placenta as well as various fetal organs were collected. Quantitative real-time polymerase chain reaction (qPCR), histopathology (hematoxylin-eosin (HE) staining) and Ziehl-Neelsen (ZN) staining were performed. Histopathological analysis revealed lesions consistent with paratuberculosis (PTB) in the mesenteric lymph node and small intestine, and ZN staining revealed the presence of acid-alcohol resistant bacilli (AARB). Histological lesions were not identified in fetal tissues. qPCR indicated that uterus, mesenteric lymph node and small intestine samples from the buffalo cow were positive for Map. In contrast, in the fetus, only samples from the digestive tract were positive for Map based on qPCR data. These results indicate that Map is present in various buffalo organs and tissues, including within the reproductive system. Therefore, the intrauterine transmission of Map may occur in buffaloes and could represent an important source of infection.

Keywords: Johne’s disease; Diagnostic; Buffaloes; Intrauterine transmission

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