Journal of Clinical & Experimental OncologyISSN: 2324-9110

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Research Article, J Clin Exp Oncol Vol: 5 Issue: 4

MDM2 Gen Polymorphisms at Acute Myeloid Leukemia Patients

Ceren Tekin1, Sibel Bayil Oğuzkan2*, Mehmet Ozaslan1, Handan Haydaroğlu2, Işik Didem Karagöz1, Ibrahim Halil Kılıç1, Selin Büdeyri2 and Mustafa Pehlivan2
1Department of Biology, Gaziantep University., Gaziantep, Turkey
2Department of Hematology Gaziantep University., Gaziantep, Turkey
Corresponding author : Sibel Bayil Oguzkan
Department of Biology, Gaziantep University, Gaziantep, Turkey
Tel: 0090 5324862521
Fax: 0090 342 3604423
E-mail: [email protected]
Received: June 23, 2016 Accepted: September 01, 2016 Published: September 07, 2016
Citation: Tekin C, Oğuzkan SB, Ozaslan M , Haydaroğlu H, Karagöz ID, et al. (2016) MDM2 Gen Polymorphisms at Acute Myeloid Leukemıa Patients. J Clin Exp Oncol 5:4. doi:10.4172/2324-9110.1000166

Abstract

Purpose: Leukemia is a malignant disease which is caused by bone marrow lymphopoietic or hematopoietic stem cells. Acute myeloid leukemia (AML) is a class of leukemia which indicates phenotypic and genotypic heterogeneity. MDM2 (Murine double minute 2 ) gene is a proto-oncogenes and previously studies in different cancer types shows that polymorphisms in MDM2 gene has connection with these cancer types.

Methods: In this study, it is aimed that to determine whether a relation between MDM2 gene 354 A/G and -410 T/G regions’s single nucleotide polymorphisms with Acute Myeloid Leukemia (AML) formation.To determine the polymorphism that turns Adenine nucleotide to guanine in the part of 354 A/G of MDM2 gene and in order to turns thymine nucleotide to guanine in the part of -410 T/G, the healthy 20 people were included to the study as a control group and 80 AML diagnosed patient. The blood which was collected from the study and control group isolated the DNA. Both two parties polymorphisms were studies RT-PCR.

Results: As a result of 354 A/G part polymorphism, it was determined that all individuals have a genotype of wild type (AA). When it was compared the MDM2 gene 354 A/G part polymorphic distribution, it was not observed any significant difference between the patient and control groups statistically (p<0.005). As a result of -410 T/G part polymorphism evaluation; the 19 of 80 patients (23.75) have wild type (TT), 25 of them (31.25%) have heterozygous (TG) genotype and 36 of them (45%) mutant (GG) genotype were determined. When it was compared to MDM2 gene -410 T/G part’s polymorphic distribution it was observed a significant difference between the patient and control groups statistically (p<0.05).

Conclusion: The MDM2 354 A/G region is not associated with acute myeloid leukemia. But the MDM2 SNP-410 in the promoter region polymorphisms and to play a role in the pathogenesis of acute myeloid leukemia has been identified. Beside this these polymorphisms could be a marker for early diagnosis and molecular analysis.

Keywords: MDM2; Acute myeloid leukemia; RT-PCR

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