Journal of Clinical & Experimental OncologyISSN: 2324-9110

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Research Article, J Clin Exp Oncol Vol: 5 Issue: 2

Micro RNA-142-5p Profile as a Predictor of Tumor Markers Regulation in Different Histological Grades of Human Breast Carcinoma

Parvathi MVS1*, Balakrishna Murthy P1 and Jagan Mohan Rao P2,3
1International Institute of Biotechnology and Toxicology, padappai, Tamil Nadu, India
2PN 14, Lakshmi Nagar Extension, Selaiyur, Chennai, Tamil Nadu, India
3Palamur Biosciences Pvt Ltd, Technology Research park, SVS medical College Campus, Yenugonda, Mahabubnagar, Telangana, India
Corresponding author :Parvathi MVS
Department of Genetic toxicology International Institute of Biotechnology and Toxicology padappai, Tamil Nadu, India-601301
Tel: +91-9884506527
E-mail: [email protected]
Received: January 18, 2016 Accepted: April 22, 2016 Published: April 26, 2016
Citation: Parvathi MVS, Balakrishna Murthy P, Jagan Mohan Rao P (2016) Micro RNA-142-5p Profile as a Predictor of Tumor Markers Regulation in Different Histological Grades of Human Breast Carcinoma. J Clin Exp Oncol 5:2. doi:10.4172/2324-9110.1000157

Abstract

Micro RNA (miRNA) is one of the kinds in non coding RNA class that regulates gene expression by translational repression, mRNA cleavage, mRNA disruption initiated by miRNA-guided rapid de-adenylation. Up- and/or down-regulated expression study of the miRNA is a good approach to study their function in cancer pathogenesis. In our previous study we understood the down regulation of polycomb gene BMI1 and its correlation with histochemical markers like ER, PR and Her2 in different histological grades of breast tumors. To derive on the identification of regulatory factor accountable for this gene regulation pattern, we tried to identify miRNA that could sway on the gene regulation with the consecutive tumor grades. To adequate this perspective we identified miR-142-5p as a regulating marker for the proliferation of human ductal invasive breast carcinoma using microarray hybridization and attempted to demonstrate the expression profile of miR-142-5p in various histological grades of human breast carcinoma using Real time-PCR amplification. The up regulation of miR-142-5p in histological Grades I, II and III predicted variation with the hormone receptor status. The miR-142-5p up regulation was vice versa with the down regulation of BMI1 gene in the tumors from Grade I to Grade III. Hence with the severity in tumor the up regulated miR-142-5p was estimated to show substantial regulatory effect on expression of the histological markers and polycomb gene BMI1. These signatures shall hold on the diagnosis and therapy for human breast carcinoma.

Keywords: MicroRNA-142-5p; BMI1 polycomb gene; Estrogen receptor; Progesterone receptor; Her2, Microarray hybridization; Real time PCR

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