Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 4 Issue: 5

The Effects of Different Echinostoma caproni Miracidial Doses on the Lutein and Beta-Carotene Content of Biomphalaria Glabrata Snails

DeGrandchamp D1*, Fried B2 and Sherma J1
1Department of Chemistry, Lafayette College, Easton, Pennsylvania, USA
2Department of Biology, Lafayette College, Easton, Pennsylvania, USA
Corresponding author : DeGrandchamp D
Dolcie DeGrandchamp, Department of Chemistry, Lafayette College, Easton, Pennsylvania, USA,
E-mail: [email protected]
Received: August 06, 2015 Accepted: October 15, 2015 Published: October 18, 2015
Citation: DeGrandchamp D, Fried B, Sherma J (2015) The Effects of Different Echinostoma caproni Miracidial Doses on the Lutein and β-Carotene Content of Biomphalaria Glabrata Snails. J Vet Sci Med Diagn 4:5. doi:10.4172/2325-9590.1000176

Abstract

The Effects of Different Echinostoma caproni Miracidial Doses on the Lutein and β-Carotene Content of Biomphalaria Glabrata Snails

The effects of 5, 25, and 40 Echinostoma caproni miracidia on the lipophilic pigment content of Biomphalaria glabrata snails was studied using reverse phase high performance thin-layer chromatography-densitometry. Analysis was done on the whole body at two and four weeks post exposure. All extraction and analysis steps were performed in minimal light in order to prevent pigment degradation. The pigments were extracted with acetone, evaporated to dryness, reconstituted using heptane, and analyzed on reverse phase silica gel HPTLC plates with a concentrating zone using a petroleum ether (37.8-53.5oC)-acetonitrile-methanol (1:1:2) mobile phase. The analysis yielded significant differences between the lutein and β-carotene content of the snails at two and four weeks post exposure and between the different miracidial doses.

Keywords: Biomphalaria glabrata; Echinostoma caproni; Miracidia; Lutein; β-carotene; Pigments; Thin-layer chromatography

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