Alpha-lipoic acid produces bone-protective effects via modulating Nox4/ROS/NF-κB and Wnt/ Lrp5/β-catenin signaling pathways in H2 O2 -treated MC3T3-E1 cells and ovariectomy rats
Hui-Jun Sun, Chao Fu, Chang-Yuan Wang, Yue Jin, Qi Liu, Qiang Meng and Ke-Xin Liu
Dalian Medical University, China
: J Regen Med
Abstract
Recently, increasing studies proved the relationship between oxidative stress and osteoporosis. And some studies have been done to detect the possible beneficial effects of Alpha-lipoic acid (ALA), a potent antioxidant, on osteoporosis in vivo and in vitro. However, the detailed mechanism(s) underlying the bone-protective action of ALA are still poorly understood. The present study aims to examine the mechanisms by which ALA produces bone-protective effects in vitro and in vivo on base of its antioxidant effects, thus the effects of ALA on H2 O2 -treated MC3T3-E1 pre-osteoblasts and ovariectomized osteoporosis rat model were investigated by using bone biomechanical testing, micro computed tomography, western blotting and qRTPCR analysis. The results showed that ALA promoted osteoblastic formation, inhibited osteoblastic apoptosis, increased OPG/RANKL ratio and enhanced bone formation in vitro and prevented bone loss in vivo. And the effects of ALA were via modulating Nox4/ROS/NF-κB and Wnt/Lrp5/β-catenin signaling pathways. The current study indicated that ALA might be beneficial for the prevention and therapy of osteoporosis clinically at the end of advanced studies.
Biography
Email: sunhuijun@dlmedu.edu.cn
