Comparative Stem Cells Therapy for regeneration of liver fibrosis
Rezk H.M, Imam H. M, and Tohamy A.F, Wassim N.H
Suez Canal University, Ismalia, Egypt Cairo University, Egypt Cairo University, Egypt Suez University,Egypt
: J Regen Med
Abstract
Background: Human umbilical cord blood is considered as a unique source for stem cells. Human umbilical cord blood contains different types of progenitor cells which could differentiate into hepatocytes. Aims: To investigate the potential of rat's liver damage repair using human umbilical cord mesenchymal stem cells. We investigated the feasibility for human umbilical cord mesenchymal stem cells in recovery from liver damage. Moreover, investigating fibrotic liver repair and using the carbon tetra chloride -induced model for liver damage in the rat. Methods: Rats were injected with 0.5 ml/kg carbon tetra chloride to induce liver damage and progressive liver fibrosis. Human umbilical cord mesenchymal stem cells were injected into the rats through the tail vein; Stem cells were transplanted at a dose of 1×106 cells/rat after 72 hours of carbon tetra chloride injection without receiving any immunosuppressant. After (6 and 8 weeks) of transplantation, blood samples were collected to assess liver functions and level of Procollagen III as a liver fibrosis marker. In addition, hepatic tissue regeneration was assessed histopathologically and immunohistochemically using antihuman monoclonal antibodies against CD34, CK19 and albumin. Results: Biochemical and histopathological analysis showed significantly increased recovery from liver damage in the transplanted group. In addition, Human umbilical cord blood stem cells trans-differentiated into functional hepatocytes in rats with hepatic injury which results in improving liver structure and function. Conclusion: Our findings suggest that transplantation of human umbilical cord mesenchymal stem cells may be a novel therapeutic approach for treating liver fibrosis. Therefore, human umbilical cord mesenchymal stem cells are a potential option for treatment of liver cirrhosis.
Biography
