Development of anti-age medications based on ethosomes for transdermal delivery of methylene blue photosensitizer

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Irina S Nikitenko, Anna G Loginova and Victor B Loschenov

Clinic of Aesthetic Cosmetology and Dermatovenerology LLC EsteMed, Russia
Kyrgyz-Russian Slavic University, Russia
Medical photonics, UAE

: J Plast Surg Cosmetol

Abstract

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MB). There are a number of publications confirming the use of MB as a drug mitochondrial action, brings an effective result in stimulating the proliferation of skin fibroblasts and slowing cellular aging. For quality assessment of ethosomal complexes we use spectroscopic methods and methods for estimating the size of ethosomes using dynamic light scattering and scanning electron microscopy. The purpose of this study is to increase the penetration depth of the photosensitizer after its application to the skin. Methodology & Theoretical Orientation: We developed a new method of transdermal delivery of methylene blue through the skin barrier. After applying the ethosomal complex to the treated (after removing the SC) and untreated pig skin, these areas were irradiated with an infrared light source. A confocal microscope was used in these areas to measure the penetration depth. Conclusion and significance: The obtained results (Figure 1) showed that there was a sufficient concentration at a depth of more than 1.1 mm, which indicates penetration into the dermis. The rate of decrease in the concentration of MS is significantly less in comparison with the generally accepted value manifested by the diffuse mechanism of distribution of the drug substance. This means that stimulating with IR light significantly increases the depth of penetration by the ethosomes Recent publications: 1. Anti-Aging Potentials of Methylene Blue for Human Skin Longevity/Z.-M. Xiong, M. O'Donovan, L. Sun et al. // Scientific Reports. 2017. Vol. 7.P. 1-12. 2 Long-term improvement on photoaging after ALA photodynamic therapy for actinic keratosis: A retrospective study / Z. Linglin, Z. Zijun, W. Peiru et al. // Photodiagnosis and Photodynamic Therapy. 2021. Vol. 33. P. 1-5. 3 The Potential Anti-Photoaging Effect of Photodynamic Therapy Using Chlorin e6-Curcumin Conjugate in UVB-Irradiated Fibroblasts and Hairless Mice / Hur, G.-H.; Ryu, A.-R.; Kim, Y.-W.; Lee, M.-Y. // Pharmaceutics 2022, 14, 968. 4 Hexyl-aminolevulinate ethosomes-mediated photodynamic therapy against acne: in vitro and in vivo analyses / W. Tai, W. Lifang, W. Yingzhe et al. //Drug Delivery and Translational Research. 2021. Vol. 12. p. 325-332. 5. The application of physical pretreatment in photodynamic therapy for skin diseases/L. Dihui, Z. Shuang, L. Jinmao et al. // Lasers in Medical Science. 2021. Vol. 36. p. 1369-1377.

Biography

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Nikitenko Irina Sergeevna is a Dermatovenerology, dermatooncologist and cosmetologist. She is the author of methods for the successful treatment of such complex diseases as acne, rosacea, psoriasis, chronic dermatoses. The author constantly takes advanced training courses abroad, and also takes annual participation in IMCAS She is a certified expert in Anti-Age Expert Swiss Group, as well as an active mentor at Merz Aesthetics. The main specialties of the doctor are anti-aging medicine, hardware cosmetology, nonsurgical facial rejuvenation, removal of age spots, neck lift, and RF-lifting. Her extensive professional experience is 11 years. So the author is looking for new ways to develop modern cosmetology, dermatology and venereology and demonstrates a new development.