Protective efficacy of carnosic acid against hydrogen peroxide (H2 O2 )-induced oxidative injury in HepG2 cells through SIRT1 pathway
Jihong Yao, Yan Hu, Ning Zhang, Qing Fan and Musen Lin
Dalian Medical University, China
: J Regen Med
Abstract
Carnosic acid (CA), found in rosemary, has been reported to have antioxidant and anti-adipogenic properties. Here, we investigate the molecular mechanism by which CA inhibits hydrogen peroxide (H2 O2 )-induced oxidative injury in HepG2 cells. Cells were pretreated with 2.5–10 µM of CA for 2 h and then exposed to 3 mM H2 O2 for an additional 4 h. CA dose dependently increased cell viability and decreased lactate dehydrogenase (LDH) activities. Pretreatment with CA completely attenuated the inhibited expression of manganese superoxide dismutase (MnSOD) and the B-cell lymphoma-extra large (BclxL) caused by H2 O2 exposure, whereas reversed the reactive oxygen species (ROS) accumulation and the increase in cleaved caspase-3. Importantly, sirtuin1 (SIRT1), a NAD+-dependent deacetylase, was significantly increased by CA. Considering the above results, we hypothesized that SIRT1 may play important roles in the protective effects of CA in the H2 O2 injury. As expected, SIRT1 suppression by a specific inhibitor of SIRT1, Ex527, significantly aggravated H2 O2 -induced increased level of cleaved caspase-3, but deeply reduced the decreased expression of MnSOD and Bcl-xL. Collectively, the present study indicated that CA can alleviate H2 O2 -induced hepatocyte oxidative and apoptotic damage through SIRT1 pathway
Biography
Email: yaojihong65@hotmail.com
