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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 4 Issue: 1

Protection against P. multocida B:2 using Recombinant 87kDa OMP Periplasmic Protein

M Bagath1,2*, Megha Kadam Bedekar1, Pallab Chaudhuri1, Varalakshmi S3 and PP Goswami1
1Genetic engineering of Bacteria Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izat Nagar, Bareilly, Uttar Pradesh-243122, India
2Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560030, India
3Dairy Microbiology, National Dairy Research Institute, Adugodi, Bangalore-560030, India
Corresponding author : Dr. Bagath M
Scientist, Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560030, India
Tel: +91-9035865536; Fax: +91-080-25711420
E-mail: [email protected]
Received: June 28, 2014 Accepted: September 01, 2014 Published: September 03, 2014
Citation: Bagath M, Megha KB, Pallab C, Varalakshmi S, Goswami P.P. (2015) Protection against P. multocida B:2 using Recombinant 87kDa OMP Periplasmic Protein. J Vet Sci Med Diagn 4:1. doi:10.4172/2325-9590.1000151

Abstract

Protection against P. multocida B: 2 using Recombinant 87kDa OMP Periplasmic Protein

To clone and express Pasteurella. multocida (P. multocida) encoding 87 kDa omp gene in prokaryotic (secretory) vector and to study the immunogenicity of expressed protein.The genomic DNA was isolated from P. multocida B:2. Specific primers were designed to suite secretory prokaryotic vector system. PCR amplified gene was cloned in pGEMT-EASY vector (Promega, USA) and subcloned into pBAD/gIIIA prokaryotic expression vector. The protein was purified and Passive mouse protection test challenge was conducted in mice.

Keywords: 87KDa; P. multocida B: 2; Secretory expression system; Passive mouse protection test; OMP

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