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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 1 Issue: 2

Utilization of Denaturing Gradient Gel Electrophoresis (DGGE) to evaluate the Intestinal Microbiota of Brown Trout Salmo trutta fario

Marisa Manzano1*, Lucilla Iacumin1, Cristina Giusto1, Francesca Cecchini1, Chiara Patthey1, Ramon Fontanillas2 and Giuseppe Comi1
1Department of Food Science, University of Udine, Italy
2Skretting Aquaculture Research Centre (ARC), Stavanger, Norway
Corresponding author : Marisa Manzano
Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy
Tel: +390432558127; Fax: +390432558130;
E-mail: [email protected]
Received: September 12, 2012 Accepted: January 07, 2013 Published: January 11, 2013
Citation: Manzano M, Iacumin L, Giusto C, Cecchini F, Patthey C, et al. (2012) Utilization of Denaturing Gradient Gel Electrophoresis (DGGE) to evaluate the Intestinal Microbiota of Brown Trout Salmo trutta fario. J Vet Sci Med Diagn 1:2. doi:10.4172/2325-9590.1000105

Abstract

Utilization of Denaturing Gradient Gel Electrophoresis (DGGE) to evaluate the Intestinal Microbiota of Brown Trout Salmo trutta fario

The microbial flora present in the intestine of the fish plays an important role, such as to break down ingested foods, or the inhibition of the colonization of the fish intestine by pathogens. Because only a small percentage of microorganisms in seawater and freshwater can be cultured and cultivated in the laboratory, molecular techniques based on 16S rDNA amplification have been developed to detect and identify culturable and nonculturable bacteria. Many studies have combined traditional methods with molecular methods to provide more detailed informations on microbial communities. The aim of this work was to evaluate the microbiota of Salmo trutta fario intestine, using PCR-Denaturing Gradient Gel Electrophoresis (DGGE), to assess the influence of a diet on the population composition.

Keywords: DNA fingerprinting; Intestinal microbiota of fish; Salmo trutta fario; PCR; DGGE; Fish diet

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