Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase and Α-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia

Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase and Α-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia

Abstract

Phytochemicals are extensively found at different levels in many medicinal plants. This work had three objectives: the first, to evaluate the total phenolic, flavonoids content, anthocyanins concentration and condensed tannins of two Tunisian medicinal plants, second, to assess their potential antioxidant activities using systems such as a, adiphenyl-b-picrylhydrazyl (DPPH• ), ABTS•+, O2 ‒• , H2 O2 scavenging activity, Hydroxyl OH• and linoleic acid inhibition and third, to screen α-amylase and α-glucosidase inhibitors from common natural products found in traditional Tunisian medicines. The present study was performed that C. flammula (Ranunculaceae family)has the highest levels of total phenols followed by P. angustifolia (Asclepiadaceae family). On the other hand, the results show that the antiradical activity (DPPH) varies from 52 to 77% for P. angustifolia and 56 to 84% for C. flammula. The antioxidant activity measurement, expressed as Trolox equivalent antioxidant capacity (TEAC), ranged from 0.33 to 1.51 mM TE/ g DW. In general, all extracts exhibited good antioxidant activity. In the present investigation, the extracts most effective in inhibiting α-amylase (C. flammula and P. angustifolia) contain appreciable amounts of condensed tannins. In addition, the extent of inhibition of α-glucosidase was related to their anthocyanins content.