Journal of Immunological Techniques & Infectious Diseases ISSN: 2329-9541

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

A flow cytometry gating strategy for profiling CD4+CD25+/high CD127-/low regulatory T cells in the Rheumatoid Arthritis scenario.

Regulatory T cells (tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human tregs are defined as CD4+CD25+/ highCD127-/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for treg profiling, useful for immunomonitoring in clinicals assessment.

Special Features

Full Text


Track Your Manuscript

Media Partners