Journal of Proteomics & EnzymologyISSN: 2470-1289

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Design of Experiments as Approach for Enhancing the Production of the Nicotinamide Co factor by Saccharomyces cervisiae

Nicotinamide adenine dinucleotide (NAD+) has crucial roles in metabolic and biosynthetic reactions. Although these cofactors have long been indispensable in analytical assays, their practical applications remain limited because of the high cost of these molecules. The aim of this work was to produce the oxidized form of cofactor efficiently and with higher concentrations. To make the production more reliable, simpler and cheaper, Saccharomyces cerevisiae was used as source of this cofactor. The production of NAD+ was improved by an optimization of the growth medium of yeast cells. The experimental program proposed on the basis of a full 23 factorial design demonstrated that the better production was achieved by the respective use of 60 g glucose/L and 30 g peptone/L in anaerobically medium. Significant amounts of NAD+ (up to 232.2 mg NAD+/ 100 g extract) were obtained in this case

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