Journal of Proteomics & EnzymologyISSN: 2470-1289

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Research Article, J Biocatal Biotransformation Vol: 3 Issue: 1

Design of Experiments as Approach for Enhancing the Production of the Nicotinamide Co factor by Saccharomyces cervisiae

Najla Ben Akacha1*, Rabaa Ben Asker1 and Mohamed Gargouri2
1Laboratory of Natural Substances,National Institute of Research and Physical and Chemical Analysis (INRAP), BiotechnopôleSidiThabet, 2020 Ariana, Tunisia
2Biocatalysis and Industrial Enzymes Group, Laboratory of Microbial Ecology and Technology, National Institute of Applied Sciences and Technology (INSAT) Tunis, University of Carthage Tunisia
Corresponding author : Najla Ben Akacha
Laboratory of Natural Substances,National Institute of Research and Physical and Chemical Analysis (INRAP),Biotechnop├┤leSidiThabet, 2020 Ariana, Tunisia
Tel: +216.71.537.666 +216.71.537.659; Fax: 216.71.537.688
E-mail: [email protected]
Received: June 17, 2014 Accepted: August 08, 2014 Published: August 25, 2014
Citation: Akacha NB, Pastor I, Asker RB, Gargouri M (2014) Design of Experiments as Approach for Enhancing the Production of the Nicotinamide Cofactor by Saccharomyces cervisiae. J Biocatal Biotransformation 3:1. doi:10.4172/2324-9099.1000116

Abstract

Nicotinamide adenine dinucleotide (NAD+) has crucial roles in metabolic and biosynthetic reactions. Although these cofactors have long been indispensable in analytical assays, their practical applications remain limited because of the high cost of these molecules. The aim of this work was to produce the oxidized form of cofactor efficiently and with higher concentrations. To make the production more reliable, simpler and cheaper, Saccharomyces cerevisiae was used as source of this cofactor. The production of NAD+ was improved by an optimization of the growth medium of yeast cells. The experimental program proposed on the basis of a full 23 factorial design demonstrated that the better production was achieved by the respective use of 60 g glucose/L and 30 g peptone/L in anaerobically medium. Signi?cant amounts of NAD+ (up to 232.2 mg NAD+/ 100 g extract) were obtained in this case

Keywords: Cofactor;Factorial design;Optimization;Yeast

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