Medical Microbiology Reports

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Evaluation of antimicrobial and antioxidant activity of Zyziphus spina-cristi acetone extract against some Pathogenic microorganisms

This Investigation was carried out in an attempt to study the antimicrobial activity of Zizyphus spina-cristi traditionally used in Yemen against some pathogenic microorganisms including Staphylococcus aureus (ATCC653-8), Pseudomonas aeruginosaMTCC2453 Mar.2001, Bacillus cereus (ATCC6633), Escherichia coli MTCC739 Mar.2001, Candida albicans(ATCC2019) & Staphylococcus epidermides(local isolate). The results indicated that the Ziziphus spina-crista acetone extract is affective against tested gram positive bacteria while don’t have any effect against tested gram negative bacteria in addition to Candida albicans. In general, all extracts given an effect on the gram positive bacteria only, The highest inhibition zone given by the high concentration of Ziziphus spina-cristi acetone extract 300mg/ml on Bacillus cereus. While the lower inhibition zone given by third concentration 100mg/ml against B.cereus. The results of the DPPH scavenging activity of acetone extract possess antioxidant activity at different concentrations with IC50 value equal to 172.077 ug / ml compared to the IC50 value of control sample (L-Ascorbic acid) that equal to 145.8838 ug / ml.

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