Advanced Biomedical Research and Innovation

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Generation of artificial pancreas by using MIN-6 cell line culture seeded in decellularized testis's rat scaffold

Objective: Type 1 diabetes is an autoimmune disease which is due to the lack of š›½ cells. Regenerative medicine offers the opportunity for curing diabetes. Native extracellular matrix (ECM) creates a reconstruction platform to replace the organs. A decellularized rats testis was used as a natural 3D scaffold in this experiment. MIN_6 š›½ cells were planted in the scaffold. Methods: The decellularized testes were prepared for in vitro analyses. Results: The decellulalarazed testes were analyzed by histological studies such as H&E and DAPI staining. SEM showed that the 3D ultra structure of the testis remained intact. In vitro studies point out that the decellularized scaffold was non_toxic and preformed a good plateform for MIN_6 š›½ cells. The DTZ staining showed cells in recellularized testis. Expression of genes (PDX1, Glut2, insulin) with RT_PCR and (PDX1,NSE,nestin)with westernblotting in recellularized testis were upregulated compare with MIN_6 š›½ cells in media culture. Insulin release from recellularized showed increasing (PĖ‚0.05) when exposed a high glucose media. Conclusion: The decellularized testis can be considered as an artificial pancrease and may help to cure type 1 diabetic pataints.

Special Features

Full Text


Track Your Manuscript

Media Partners