Preparation and Evaluation of Rosmarinic Acid Liposomes as an Ocular Delivery System
Background: Cataract is considered the leading cause of visual impairment. Recently, Rosmarinic Acid (RA) shows potential benefits for pharmacologic treatment of cataract. Unfortunately, due to its low permeation to the lens, liposomes have been used to overcome of its solubility and improve efficiency.
Methods: In the current study, different formulations of liposome incorporating Sodium Deoxy Cholate (SDC) were prepared by ethanol-injection method. Liposomes were characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), UV-visible spectroscopy, encapsulation efficiency, in vitro drug release, and release kinetics. Finally, stability of liposomes was performed.
Results and discussion: RA liposome showed variable Nano metric size distribution from 136 nm to 44 nm and demonstrates spherical morphology. The highest loading efficiency of liposomal RA was 52% and illustrated 73% release its payload after 9 hours. Liposomes show good stability at 5, and 25°C following 6 months.
Conclusion: The results illustrated that RA liposome would be a great tool to enhance the penetration of hydrophobic agent through the cornea and improve therapeutic outcomes. In vivo experiments should be confirmed our current in vitro results.