Production of Hydroxyapatite Nanoparticles Using Tricalcium- Phosphate by Alkanindiges illinoisensis

Production of Hydroxyapatite Nanoparticles Using Tricalcium- Phosphate by Alkanindiges illinoisensis

The general purpose of this study was to examine the role of urease and phosphatase in production of hydroxyapatite (HA) nanocrystal that was performed using two different media of precipitating and Pikovskaya (PVK), respectively. We evaluated properties of HA by means of X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM) an equipped with energy dispersive X-ray spectroscopy (EDX). Among bacterial strains isolated from nature, only two strains C17 and C21 had the ability to produce HA nanocrystal. These strains were phosphatase-positive and produced HA only in the presence of tricalcium-phosphate as an insoluble mineral phosphorus, therefore phosphatase have a direct role in the HA production. In the other hand, none of strains which were urease-positive had any ability to produce HA, therefore urease has no role in HA production. Crystallite particle size was determined from XRD data using Scherrer formula that was sub- 25-nm. Based on data obtained, the strain C17 selected as the best HA-producing strain. The BLAST analysis showed that partial 16S rRNA sequence of this isolate is more than 99% identical of Alkanindiges illinoisensis that was deposited in GenBank with accession number JX666243. The aim of this study was to find easier and cheaper ways for HA production using tricalcium-phosphate as an inorganic phosphate instead of glycerol-2- phosphate (G-2-P) that was used by previous researchers.