Proliferation and Promoting the Apoptosis
Discovery of cell apoptosis SW620 cells in logarithmic growth phase (1 × 105 cells/ ml) were invested in 6 bottles with volume of 50 ml, 1 ml for each bottle. After cell adhesion, 1 ml of emodin with 5 different attention (10 μmol/ L, 20 μmol/ L, 40 μmol/ L, 80 μmol/ L, 160 μmol/ L) was added to the bottle. The control group was added with 1 ml of RPMI 1640 medium. The culture was performed at 37?, with 5 CO2 and impregnated moisture for 48 h. The admixture was centrifuged at 256 Xg for 10 min. The cells were collected, followed by washing using Phosphate Buffer Saline (PBS) (pH 7.4) for 3 times. Eventually the propidium staining was performed for 30 min, and the apoptosis of SW620 cells was measured by inflow cytometry.